منابع مشابه
Biogenesis of cytochrome c in Neurospora crassa.
1. Precipitating antibodies specific for apocytochrome c and holocytochrome c, respectively, were employed to study synthesis and intracellular transport of cytochrome c in Neurospora in vitro. 2. Apocytochrome c as well as holocytochrome c were found to be synthesized in a cell-free homogenate. A precursor product relationship between the two components is suggested by kinetic experiments. 3. ...
متن کاملA mutant of Neurospora crassa deficient in cytochrome c heme lyase activity cannot import cytochrome c into mitochondria.
The nuclear cyt-2-1 mutant of Neurospora crassa is characterized by a gross deficiency of cytochrome c (Bertrand, H., and Collins, R. A. (1978) Mol. Gen. Genet. 166, 1-13). The mutant produces mRNA that can be translated into apocytochrome c in vitro. Apocytochrome c is also synthesized in vivo in cyt-2-1, but it is rapidly degraded and thus does not accumulate in the cytosol. Mitochondria from...
متن کاملThe primary structure of cytochrome c1 from Neurospora crassa.
The primary structure of the cytochrome c1 subunit of ubiquinol-cytochrome-c reductase from mitochondria of Neurospora crassa was determined by sequencing the cDNA of a bank cloned in Escherichia coli. From the coding region the sequence of 332 amino acids, corresponding to the molecular mass of 36,496 Da, was derived for the precursor protein. The mature protein, the N terminus of which was pr...
متن کاملA nuclear mutant of Neurospora crassa lacking subunit 1 of cytochrome c oxidase.
A new, nuclear, cytochrome-deficient mutant of Neurospora crassa has been isolated. The mutant is characterized by its slow growth rate, high dependence on the alternate oxidase pathway of respiration, absence of spectrally detectable cytochrome aa3, and low levels of cytochrome c oxidase activity. The amount of cytochrome b appears to be normal in the mutant whereas the amount of cytochrome c ...
متن کاملCytochrome c oxidase in Neurospora crassa contains myristic acid covalently linked to subunit 1.
Radiolabel from [3H]myristic acid was incorporated by Neurospora crassa into the core catalytic subunit 1 of cytochrome c oxidase (EC 1.9.3.1), as indicated by immunoprecipitation. This modification of the subunit, which was specific for myristic acid, represents an uncommon type of myristoylation through an amide linkage at an internal lysine, rather than an N-terminal glycine. The [3H]myrista...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1966
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)96512-9